What I would do is give the spores some regular culture media so they wake up and start growing, then spray that on, and once they are exposed to oxygen, they will start forming the mycelium and start sporulating.
Your choices are PDB (PDA, basically, but no agar), PDY, MDB, or similar.
here's the recipe to accommodate a wide variety of yeasts and fungi, for one liter:
- 20 grams dextrose
- 4 grams potato starch
- 3 grams MgSO4, the 7 hydrate (epsom salt works well)
- 0.5-1.0 grams yeast extract (for the b vitamins, for the cell walls)
- .5 tablets of an all-in-one vitamin pill (for misc vitamins and trace elements)
Make that up, and boil (use pressure boil if you have it) for 30 minutes (or autoclave at 121C, per usual lab protocol). Then chill, and inoculate at about 75F. If you want to make the agar version for slants, add 15 grams agar powder to the above.
You can use store bought potato flakes for the starch, it works better, actually. Or whole potatoes (different amounts, of course). And for the dextrose, that's just corn sugar. You can find it at a homebrew place. Yeast extract is nutritional yeast, you can get it at a bakery supply place or supplement store.
You can also propagate most yeasts and molds on this medium. Propagate, not continuously culture. For continuous fermentation, you need something else. Just in case you were wondering
Let me know if you get stuck.
Oh, once you do inoculate, use up the spray or toss. Don't make up a large solution. After the culture mix becomes turbid (2-3 days), it will rather quickly kill itself with all the toxins it will produce. useful life is maybe 3-4 days.