Hi Lloyd,
I've read in the Home Cheesemaking book by Rikki Carroll that when making mother cultures you want to totally sterilize the milk, so the starter culture you add in later are the *only* bacteria that are in the milk. Vat pasteurized milk, as I understand it, does not totally kill all the bacteria, so I think you are correct in what you did. It's a bit of a bummer that the milk was heat treated twice, but since there is not that much of it added in to your cheese it should not be a big deal? So I'm thinking your effort was not wasted, but then I'm not an expert on the subject as thus far I've only used direct-set starter cultures. --Susan