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Hello again,

I made a mesophilic MC about a month ago.  I froze a chunk of it in cubes and used a chunk fresh.  I am trying to use the frozen culture for the first time to make creme fraiche and now at about 18 hours since inoculating 1/2 L of cream with 2 oz frozen MC I see no coagulation.

I assume this means my frozen culture is dead, but what might have caused this?  I froze and quickly transferred cubes to a plastic bag.

Thanks!

what temp, and how does it smell and what culture?

Aromatic B from Abiasa.  It smells a bit sour creamy.  It was around 70-72F.  Is it worth waiting a bit longer?  I have some pH strips but they only do a limited range (4.6-6.2) so not sure if they will give me an accurate reading?

That is low for Aromatic B. You need 76-78F. Yes, your culture is working fine. Raise the temp a bit and wait 12 hours or so. temp makes a huge difference.

okay thanks i'll see what happens.

when i used the culture from the unfrozen mother it was just so much faster so i thought something might be wrong.